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jun cdna  (OriGene)


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    Structured Review

    OriGene jun cdna
    Jun Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+c-jun+cdna/c-Jun+(JUN)+(NM_002228)+Human+Untagged+Clone/pmc12771353-56-12-14
    Average 94 stars, based on 6 article reviews
    jun cdna - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Knockdown:

    Article Title: Protein kinase C? regulation of translocator protein (18 kDa) Tspo gene expression is mediated through a MAPK pathway targeting STAT3 and c-Jun transcription factors
    Article Snippet: ERK2 shRNA plasmid treatment MA-10 cells were seeded at 150,000 cells per well in 6-well plates for 24 h before being transfected for an additional 72 h with 800 ng of shRNA ERK2 plasmid or pKD-negative control-v1 (Millipore, Lake Placid, NY) using Fugene 6 (Roche), followed by collection for protein analysis. .. Target gene knockdown was verified by immunoblot analysis of cell extracts. c-Jun overexpression NIH-3T3 cells were seeded in 12 well plates at 10,000 cells/well for 24 h before transfection with 10 ng of either pCMV-6/c-Jun containing Homo sapiens c-Jun cDNA (bearing >93% homology with mouse c-Jun; Origene, Rockville, MD), or empty pCMV-6 vector as a control along with pGL3-805 or pGL3-805m (containing a double mutation at AP1 and Ets sites) or pGL3- Basic as a control. ..

    Article Title: Protein kinase C epsilon regulation of translocator protein (18 kDa) Tspo gene expression is mediated through a MAPK pathway targeting STAT3 and c-Jun transcription factors.
    Article Snippet: MA-10 cells were seeded at 150000 cells per well in six-well plates for 24 h before being transfected for an additional 72 h with 800 ng of shRNA ERK2 plasmid or pKD-negative control-v1 (Millipore, Lake Placid, NY) using Fugene 6 (Roche), followed by collection for protein analysis. .. Target gene knockdown was verified by immunoblot analysis of cell extracts. c-Jun Overexpression.NIH-3T3 cells were seeded in 12 well plates at 10000 cells/well for 24 h before transfection with 10 ng of either pCMV-6/c-Jun containing H. sapiens c-Jun cDNA (bearing>93%homologywithmouse c-Jun;Origene,Rockville, MD) or empty pCMV-6 vector as a control alongwith pGL3-805 or pGL3-805-m (containing a double mutation at AP1 and Ets sites) or pGL3-Basic as a control. ..

    Western Blot:

    Article Title: Protein kinase C? regulation of translocator protein (18 kDa) Tspo gene expression is mediated through a MAPK pathway targeting STAT3 and c-Jun transcription factors
    Article Snippet: ERK2 shRNA plasmid treatment MA-10 cells were seeded at 150,000 cells per well in 6-well plates for 24 h before being transfected for an additional 72 h with 800 ng of shRNA ERK2 plasmid or pKD-negative control-v1 (Millipore, Lake Placid, NY) using Fugene 6 (Roche), followed by collection for protein analysis. .. Target gene knockdown was verified by immunoblot analysis of cell extracts. c-Jun overexpression NIH-3T3 cells were seeded in 12 well plates at 10,000 cells/well for 24 h before transfection with 10 ng of either pCMV-6/c-Jun containing Homo sapiens c-Jun cDNA (bearing >93% homology with mouse c-Jun; Origene, Rockville, MD), or empty pCMV-6 vector as a control along with pGL3-805 or pGL3-805m (containing a double mutation at AP1 and Ets sites) or pGL3- Basic as a control. ..

    Article Title: Protein kinase C epsilon regulation of translocator protein (18 kDa) Tspo gene expression is mediated through a MAPK pathway targeting STAT3 and c-Jun transcription factors.
    Article Snippet: MA-10 cells were seeded at 150000 cells per well in six-well plates for 24 h before being transfected for an additional 72 h with 800 ng of shRNA ERK2 plasmid or pKD-negative control-v1 (Millipore, Lake Placid, NY) using Fugene 6 (Roche), followed by collection for protein analysis. .. Target gene knockdown was verified by immunoblot analysis of cell extracts. c-Jun Overexpression.NIH-3T3 cells were seeded in 12 well plates at 10000 cells/well for 24 h before transfection with 10 ng of either pCMV-6/c-Jun containing H. sapiens c-Jun cDNA (bearing>93%homologywithmouse c-Jun;Origene,Rockville, MD) or empty pCMV-6 vector as a control alongwith pGL3-805 or pGL3-805-m (containing a double mutation at AP1 and Ets sites) or pGL3-Basic as a control. ..

    Over Expression:

    Article Title: Protein kinase C? regulation of translocator protein (18 kDa) Tspo gene expression is mediated through a MAPK pathway targeting STAT3 and c-Jun transcription factors
    Article Snippet: ERK2 shRNA plasmid treatment MA-10 cells were seeded at 150,000 cells per well in 6-well plates for 24 h before being transfected for an additional 72 h with 800 ng of shRNA ERK2 plasmid or pKD-negative control-v1 (Millipore, Lake Placid, NY) using Fugene 6 (Roche), followed by collection for protein analysis. .. Target gene knockdown was verified by immunoblot analysis of cell extracts. c-Jun overexpression NIH-3T3 cells were seeded in 12 well plates at 10,000 cells/well for 24 h before transfection with 10 ng of either pCMV-6/c-Jun containing Homo sapiens c-Jun cDNA (bearing >93% homology with mouse c-Jun; Origene, Rockville, MD), or empty pCMV-6 vector as a control along with pGL3-805 or pGL3-805m (containing a double mutation at AP1 and Ets sites) or pGL3- Basic as a control. ..

    Article Title: Protein kinase C epsilon regulation of translocator protein (18 kDa) Tspo gene expression is mediated through a MAPK pathway targeting STAT3 and c-Jun transcription factors.
    Article Snippet: MA-10 cells were seeded at 150000 cells per well in six-well plates for 24 h before being transfected for an additional 72 h with 800 ng of shRNA ERK2 plasmid or pKD-negative control-v1 (Millipore, Lake Placid, NY) using Fugene 6 (Roche), followed by collection for protein analysis. .. Target gene knockdown was verified by immunoblot analysis of cell extracts. c-Jun Overexpression.NIH-3T3 cells were seeded in 12 well plates at 10000 cells/well for 24 h before transfection with 10 ng of either pCMV-6/c-Jun containing H. sapiens c-Jun cDNA (bearing>93%homologywithmouse c-Jun;Origene,Rockville, MD) or empty pCMV-6 vector as a control alongwith pGL3-805 or pGL3-805-m (containing a double mutation at AP1 and Ets sites) or pGL3-Basic as a control. ..

    Transfection:

    Article Title: Protein kinase C? regulation of translocator protein (18 kDa) Tspo gene expression is mediated through a MAPK pathway targeting STAT3 and c-Jun transcription factors
    Article Snippet: ERK2 shRNA plasmid treatment MA-10 cells were seeded at 150,000 cells per well in 6-well plates for 24 h before being transfected for an additional 72 h with 800 ng of shRNA ERK2 plasmid or pKD-negative control-v1 (Millipore, Lake Placid, NY) using Fugene 6 (Roche), followed by collection for protein analysis. .. Target gene knockdown was verified by immunoblot analysis of cell extracts. c-Jun overexpression NIH-3T3 cells were seeded in 12 well plates at 10,000 cells/well for 24 h before transfection with 10 ng of either pCMV-6/c-Jun containing Homo sapiens c-Jun cDNA (bearing >93% homology with mouse c-Jun; Origene, Rockville, MD), or empty pCMV-6 vector as a control along with pGL3-805 or pGL3-805m (containing a double mutation at AP1 and Ets sites) or pGL3- Basic as a control. ..

    Article Title: Complement-mediated regulation of the interleukin 17A axis is a central genetic determinant of the severity of experimental allergic asthma
    Article Snippet: Cells were transfected with 0.1 μg per well of an AP-1.Luciferase (Stratagene) reporter vector using FuGENE HD (Roche). .. Some cells were transfected with a 1096 bp fragment of the A/J Il23a promoter region inserted into pGL3 (Promega) in combination with 50 ng of either empty pcDNA3.1 (Invitrogen), or human c-Jun cDNA (#SC118762, Origene), or mouse c-Fos cDNA (#MC203181, Origene). .. Cells were stimulated for 24 h (HDM used at 10 μg/ml) and lysed for luciferase assay (Promega).

    Article Title: Protein kinase C epsilon regulation of translocator protein (18 kDa) Tspo gene expression is mediated through a MAPK pathway targeting STAT3 and c-Jun transcription factors.
    Article Snippet: MA-10 cells were seeded at 150000 cells per well in six-well plates for 24 h before being transfected for an additional 72 h with 800 ng of shRNA ERK2 plasmid or pKD-negative control-v1 (Millipore, Lake Placid, NY) using Fugene 6 (Roche), followed by collection for protein analysis. .. Target gene knockdown was verified by immunoblot analysis of cell extracts. c-Jun Overexpression.NIH-3T3 cells were seeded in 12 well plates at 10000 cells/well for 24 h before transfection with 10 ng of either pCMV-6/c-Jun containing H. sapiens c-Jun cDNA (bearing>93%homologywithmouse c-Jun;Origene,Rockville, MD) or empty pCMV-6 vector as a control alongwith pGL3-805 or pGL3-805-m (containing a double mutation at AP1 and Ets sites) or pGL3-Basic as a control. ..

    Article Title: Complement-mediated regulation of the IL-17A axis is a central genetic determinant of the severity of experimental allergic asthma.
    Article Snippet: Cells were transfected with AP-1 luciferase reporter vector (0.1 μg per well; Stratagene) through the use of FuGENE HD (Roche). .. Some cells were transfected with a 1,096–base pair fragment of the A/J Il23a promoter region inserted into the pGL3 vector (Promega) in combination with 50 ng empty pcDNA3.1 (Invitrogen), human c-Jun cDNA (SC118762; Origene) or mouse c-Fos cDNA (MC203181; Origene). .. Cells were stimulated for 24 h with HDM at a concentration of 10 μg/ml and were lysed for luciferase assay (Promega).

    Article Title: Protein kinase C? regulation of translocator protein (18 kDa) Tspo gene expression is mediated through a MAPK pathway targeting STAT3 and c-Jun transcription factors
    Article Snippet: .. NIH-3T3 cells were seeded in 12 well plates at 10,000 cells/well for 24 h before transfection with 10 ng of either pCMV-6/c-Jun containing Homo sapiens c-Jun cDNA (bearing >93% homology with mouse c-Jun; Origene, Rockville, MD), or empty pCMV-6 vector as a control along with pGL3-805 or pGL3-805m (containing a double mutation at AP1 and Ets sites) or pGL3- Basic as a control. ..

    Control:

    Article Title: Protein kinase C? regulation of translocator protein (18 kDa) Tspo gene expression is mediated through a MAPK pathway targeting STAT3 and c-Jun transcription factors
    Article Snippet: ERK2 shRNA plasmid treatment MA-10 cells were seeded at 150,000 cells per well in 6-well plates for 24 h before being transfected for an additional 72 h with 800 ng of shRNA ERK2 plasmid or pKD-negative control-v1 (Millipore, Lake Placid, NY) using Fugene 6 (Roche), followed by collection for protein analysis. .. Target gene knockdown was verified by immunoblot analysis of cell extracts. c-Jun overexpression NIH-3T3 cells were seeded in 12 well plates at 10,000 cells/well for 24 h before transfection with 10 ng of either pCMV-6/c-Jun containing Homo sapiens c-Jun cDNA (bearing >93% homology with mouse c-Jun; Origene, Rockville, MD), or empty pCMV-6 vector as a control along with pGL3-805 or pGL3-805m (containing a double mutation at AP1 and Ets sites) or pGL3- Basic as a control. ..

    Article Title: Protein kinase C epsilon regulation of translocator protein (18 kDa) Tspo gene expression is mediated through a MAPK pathway targeting STAT3 and c-Jun transcription factors.
    Article Snippet: MA-10 cells were seeded at 150000 cells per well in six-well plates for 24 h before being transfected for an additional 72 h with 800 ng of shRNA ERK2 plasmid or pKD-negative control-v1 (Millipore, Lake Placid, NY) using Fugene 6 (Roche), followed by collection for protein analysis. .. Target gene knockdown was verified by immunoblot analysis of cell extracts. c-Jun Overexpression.NIH-3T3 cells were seeded in 12 well plates at 10000 cells/well for 24 h before transfection with 10 ng of either pCMV-6/c-Jun containing H. sapiens c-Jun cDNA (bearing>93%homologywithmouse c-Jun;Origene,Rockville, MD) or empty pCMV-6 vector as a control alongwith pGL3-805 or pGL3-805-m (containing a double mutation at AP1 and Ets sites) or pGL3-Basic as a control. ..

    Article Title: Protein kinase C? regulation of translocator protein (18 kDa) Tspo gene expression is mediated through a MAPK pathway targeting STAT3 and c-Jun transcription factors
    Article Snippet: .. NIH-3T3 cells were seeded in 12 well plates at 10,000 cells/well for 24 h before transfection with 10 ng of either pCMV-6/c-Jun containing Homo sapiens c-Jun cDNA (bearing >93% homology with mouse c-Jun; Origene, Rockville, MD), or empty pCMV-6 vector as a control along with pGL3-805 or pGL3-805m (containing a double mutation at AP1 and Ets sites) or pGL3- Basic as a control. ..

    Mutagenesis:

    Article Title: Protein kinase C? regulation of translocator protein (18 kDa) Tspo gene expression is mediated through a MAPK pathway targeting STAT3 and c-Jun transcription factors
    Article Snippet: ERK2 shRNA plasmid treatment MA-10 cells were seeded at 150,000 cells per well in 6-well plates for 24 h before being transfected for an additional 72 h with 800 ng of shRNA ERK2 plasmid or pKD-negative control-v1 (Millipore, Lake Placid, NY) using Fugene 6 (Roche), followed by collection for protein analysis. .. Target gene knockdown was verified by immunoblot analysis of cell extracts. c-Jun overexpression NIH-3T3 cells were seeded in 12 well plates at 10,000 cells/well for 24 h before transfection with 10 ng of either pCMV-6/c-Jun containing Homo sapiens c-Jun cDNA (bearing >93% homology with mouse c-Jun; Origene, Rockville, MD), or empty pCMV-6 vector as a control along with pGL3-805 or pGL3-805m (containing a double mutation at AP1 and Ets sites) or pGL3- Basic as a control. ..

    Article Title: Protein kinase C epsilon regulation of translocator protein (18 kDa) Tspo gene expression is mediated through a MAPK pathway targeting STAT3 and c-Jun transcription factors.
    Article Snippet: MA-10 cells were seeded at 150000 cells per well in six-well plates for 24 h before being transfected for an additional 72 h with 800 ng of shRNA ERK2 plasmid or pKD-negative control-v1 (Millipore, Lake Placid, NY) using Fugene 6 (Roche), followed by collection for protein analysis. .. Target gene knockdown was verified by immunoblot analysis of cell extracts. c-Jun Overexpression.NIH-3T3 cells were seeded in 12 well plates at 10000 cells/well for 24 h before transfection with 10 ng of either pCMV-6/c-Jun containing H. sapiens c-Jun cDNA (bearing>93%homologywithmouse c-Jun;Origene,Rockville, MD) or empty pCMV-6 vector as a control alongwith pGL3-805 or pGL3-805-m (containing a double mutation at AP1 and Ets sites) or pGL3-Basic as a control. ..

    Article Title: Protein kinase C? regulation of translocator protein (18 kDa) Tspo gene expression is mediated through a MAPK pathway targeting STAT3 and c-Jun transcription factors
    Article Snippet: .. NIH-3T3 cells were seeded in 12 well plates at 10,000 cells/well for 24 h before transfection with 10 ng of either pCMV-6/c-Jun containing Homo sapiens c-Jun cDNA (bearing >93% homology with mouse c-Jun; Origene, Rockville, MD), or empty pCMV-6 vector as a control along with pGL3-805 or pGL3-805m (containing a double mutation at AP1 and Ets sites) or pGL3- Basic as a control. ..

    Clone Assay:

    Article Title: Proteogenomics of different urothelial bladder cancer stages reveals distinct molecular features for papillary cancer and carcinoma in situ
    Article Snippet: .. Whole-length RBPMS, c-Fos, and c-Jun cDNA clones were purchased from Origene. .. After confirming their sequences by sanger-sequencing, RBPMS was amplified and subcloned into pcDNA3.1-FLAG, pcDNA3.1-Myc, or pCDNA3.1-HA vector using recombinant DNA technology and were confirmed via sequencing.



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    Figure 8 C5a-induced IL-10 regulates IL-23 production by engaging Jnk and AP-1. (a) IL-10 production by medium- or HDM- treated BMDCs (BALB/c) in the presence of medium alone (Ctrl) or recombinant C3a (rC3a) or C5a (rC5a). *P < 0.05 and **P < 0.001 (one-way ANOVA). (b) IL-23 production by medium- or HDM- treated BMDCs (BALB/c) with (rC5a) or without (Ctrl) recombinant C5a in the presence of IgG1, anti-IL-10 (α-IL-10) or recombinant IL-10 (rIL-10). (c,d) Production of IL-23 (c) and IL-10 (d) by BMDCs (BALB/c) pretreated for 1 h with dimethyl sulfoxide (DMSO), SP600125, AS602868 or rapamycin, then treated with medium or HDM (100 μg/ml) in the presence (rC5a) or absence (Ctrl) of recombinant C5a. (e) Immunoblot analysis of c-Jun phosphorylated at Ser63 (p-c-Jun (Ser63)) and total c-Jun in medium- or C5a-treated RAW264.7 cells. (f) AP-1 activity in RAW264.7 cells treated with medium or various combinations of HDM, recombinant C5a and SP600125 (SP). (g) AP-1 activity in RAW264.7 cells stimulated with medium or various combinations of HDM, recombinant C5a, IgG1, anti-IL-10 and recombinant IL-10. (h) Il23a promoter activity in RAW264.7 cells cotransfected with pcDNA3.1 vector alone (p-cDNA) or vector containing c-Jun cDNA (p-c-Jun) or c-Fos cDNA (p-c-Fos), then treated with medium or HDM. (i) Jun and Fos mRNA in medium- or HDM-treated A/J and C3H/HeJ BMDCs, presented relative to S14 expression. Results are representative of two to three independent experiments (mean and s.e.m. of four to eight individual samples).

    Journal: Nature immunology

    Article Title: Complement-mediated regulation of the IL-17A axis is a central genetic determinant of the severity of experimental allergic asthma.

    doi: 10.1038/ni.1926

    Figure Lengend Snippet: Figure 8 C5a-induced IL-10 regulates IL-23 production by engaging Jnk and AP-1. (a) IL-10 production by medium- or HDM- treated BMDCs (BALB/c) in the presence of medium alone (Ctrl) or recombinant C3a (rC3a) or C5a (rC5a). *P < 0.05 and **P < 0.001 (one-way ANOVA). (b) IL-23 production by medium- or HDM- treated BMDCs (BALB/c) with (rC5a) or without (Ctrl) recombinant C5a in the presence of IgG1, anti-IL-10 (α-IL-10) or recombinant IL-10 (rIL-10). (c,d) Production of IL-23 (c) and IL-10 (d) by BMDCs (BALB/c) pretreated for 1 h with dimethyl sulfoxide (DMSO), SP600125, AS602868 or rapamycin, then treated with medium or HDM (100 μg/ml) in the presence (rC5a) or absence (Ctrl) of recombinant C5a. (e) Immunoblot analysis of c-Jun phosphorylated at Ser63 (p-c-Jun (Ser63)) and total c-Jun in medium- or C5a-treated RAW264.7 cells. (f) AP-1 activity in RAW264.7 cells treated with medium or various combinations of HDM, recombinant C5a and SP600125 (SP). (g) AP-1 activity in RAW264.7 cells stimulated with medium or various combinations of HDM, recombinant C5a, IgG1, anti-IL-10 and recombinant IL-10. (h) Il23a promoter activity in RAW264.7 cells cotransfected with pcDNA3.1 vector alone (p-cDNA) or vector containing c-Jun cDNA (p-c-Jun) or c-Fos cDNA (p-c-Fos), then treated with medium or HDM. (i) Jun and Fos mRNA in medium- or HDM-treated A/J and C3H/HeJ BMDCs, presented relative to S14 expression. Results are representative of two to three independent experiments (mean and s.e.m. of four to eight individual samples).

    Article Snippet: Some cells were transfected with a 1,096–base pair fragment of the A/J Il23a promoter region inserted into the pGL3 vector (Promega) in combination with 50 ng empty pcDNA3.1 (Invitrogen), human c-Jun cDNA (SC118762; Origene) or mouse c-Fos cDNA (MC203181; Origene).

    Techniques: Recombinant, Western Blot, Activity Assay, Plasmid Preparation, Expressing